纯度

> 60 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to EGFL6-His coated plates (5μg/mL and 100μL/well), approximately >40% will adhere specifically after 30 minutes at 37℃.

蛋白构建

A DNA sequence encoding the human EGFL6 (NP_056322.2)(Met1-Asp553) was expressed with a C-terminal polyhistidine tag.

NP No.

表达宿主

Baculovirus-Insect Cells

种属

Human

预测 N 端

Asn 22

分子量

The secreted recombinant human EGFL6 consists of 543 amino acids and predicts a molecular mass of 60.5 KDa. The apparent molecular mass of the protein is approximately 62 KDa in SDS-PAGE under reducing conditions due to glycosylation.

缓冲液

Lyophilized from sterile 20mM PB, 500mM NaCl, 10% Glycerol, pH7.5.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.