纯度

> 95 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1. Immobilized EGF Protein, Human, Recombinant (ECD), HPLC-verified (Cat:10605-HNAE) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag)(Cat:29662-H02B) in functional ELISA.
2. Immobilized Anti-EGFR(MK)-IgG1 Antibody (Cetuximab)(Cat:68069-H001) at 2 μg/mL (100 μL/well) can bind EGFR Protein, Human, Recombinant (Isoform Viii, hFc Tag)(Cat:29662-H02B), the EC₅₀ is 4-13 ng/mL.

蛋白构建

A DNA sequence encoding the human EGFR (NP_001333870.1) (Met1-Lys375) was expressed with the Fc region of human IgG1 at the C-terminus.

NP No.

表达宿主

Baculovirus-Insect Cells

种属

Human

预测 N 端

Leu 25

分子量

The recombinant human EGFR consists of 589 amino acids and predicts a molecular mass of 65.1 kDa.

缓冲液

Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.