纯度

> 90 % as determined by SDS-PAGE.

内毒素

<1.0 EU per μg protein as determined by the LAL method.

生物活性

Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 0.1-0.6 ng/mL

蛋白构建

A DNA sequence encoding the human FGFR1 alpha (IIIb) (NP_056934.2) (Met1-Lys310 and Ala359-Glu374 linked by FGFR1 IIIb Ig domain (AAB19502.1) (His1-Pro47)) was expressed with the Fc region of human IgG1 at the C-terminus.

NP No.

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Arg 22

分子量

The recombinant human FGFR1 alpha (IIIb) consists of 590 amino acids and predicts a molecular mass of 65.7 kDa. As a result of glycosylation, it migrates as an approximately 113.3 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.