纯度

> 90 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

Measured by its binding ability in a functional ELISA .
1. Immobilized mouse at 10 μg/ml (100 μl/well) can bind mouse FGFR5. The EC₅₀ of mouse FGFR5 is 0.34 μg/ml .
2. Immobilized human FGF1 at 10 μg/ml (100 μl/well) can bind mouse FGFR5 with a linear range of 0.08-2 μg/ml .
3. Immobilized human bFGF at 5 μg/ml (100 μl/well) can bind mouse FGFR5. The EC₅₀ of mouse FGFR5 is 0.22 μg/ml .

蛋白构建

A DNA sequence encoding the extracellular domain of mouse FGFRL1 (NP_473412.1) (Met 1-Pro 374) precursor was expressed, with a C-terminal polyhistidine tag.

NP No.

表达宿主

HEK293 Cells

种属

Mouse

预测 N 端

Ala 21

分子量

The secreted recombinant mouse FGFRL1 consists of 365 amino acids and has a calculated molecular mass of 40.4 kDa. The apparent molecular mass of rmFGFRL1 is approximately 55-60 kDa in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.