纯度

> 67 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.).
The ED₅₀ for this effect is typically 0.02-0.1 μg/mL in the presence of 14 ng/mL human IGFII.

蛋白构建

A DNA sequence encoding the rhesus IGFBP2 (XP_001087071.1) (Met1-Gln324) was expressed, fused with a polyhistidine tag at the C-terminus.

NP No.

表达宿主

HEK293 Cells

种属

Rhesus

预测 N 端

Glu 38

分子量

The recombinant rhesus IGFBP2 comprises 300 amino acids and has a calculated molecular mass of 32.8 KDa.

缓冲液

Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.