纯度

> 95 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

1. Immobilized human PGF-mFc at 10 μg/mL (100 μL/well) can bind human VEGFR1-His (Cat:10136-H08H). The EC₅₀ of human VEGFR1-His (Cat:10136-H08H) is 0.19-0.43 μg/mL.
2. Labeled biotin to PLGF / PGF Protein, Human, Recombinant (Fc Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PLGF / PGF Protein, Human, Recombinant (Fc Tag), Biotinylated (Cat. 10274-H05H-B) bound to Conbercept was 4.5 nM.

蛋白构建

A DNA sequence encoding the human PGF (NP_002623.2) (Met1-Arg170) was expressed with the Fc region of mouse IgG1 at the C-terminus.

NP No.

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Leu 19

分子量

The recombinant human PGF consists 386 amino acids and predicts a molecular mass of 43.7 kDa.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.