纯度

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

内毒素

< 5 EU per mg of the protein.

生物活性

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is typically 3-30 pg/mL. The specific activity of recombinant human TNF-alpha is >36,000IU/μg, which is calibrated against the human TNF-alpha WHO International Standard (NIBSC code: 12/154).

蛋白构建

A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val77-Leu233) was expressed with an initial Met at the N-terminus.

NP No.

表达宿主

E. coli

种属

Human

预测 N 端

Met

分子量

The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.

缓冲液

Supplied as sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.

运输方式

Liquid. It is shipped out with blue ice.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.