纯度

> 88 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV).
The ED₅₀ for this effect is typically 2-8 pg/mL.

蛋白构建

A DNA sequence encoding the human IFNα 4a (NP_066546.1)) (Met 1-Asp 189) was fused with a polyhistidine tag at the C-terminus.

NP No.

表达宿主

Baculovirus-Insect Cells

种属

Human

预测 N 端

Cys 24

分子量

The recombinant human IFNα4a consists of 176 amino acids and predicts a molecular mass of 20.7 kDa. It migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.