纯度

> 90 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

1. Measured by its ability to inhibit rh IFNβ mediated protection of WISH Human amnion cells infected with vesicular stomatitis virus (VSV) to viral lysis. The EC₅₀ for this effect is typically 0.2-1.2 μg/mL.
2. Measured by its binding ability in a functional ELISA. Immobilized PPICZa-IFNA2 (Cat:13833-HNAY) at 10 μg/mL (100 μL/well) can bind IFNaR2-Fc (Cat:10359-H02H), the EC₅₀ of IFNaR2-Fc (Cat:10359-H02H) is 60-200 ng/mL.

蛋白构建

A DNA sequence encoding the extracellular domain of human IFNAR2 isoform a (NP_997468.1) (Met 1-Lys 243) was fused with the Fc region of human IgG1 at the C-terminus.

NP No.

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Ile 27

分子量

The recombinant human IFNAR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 458 amino acids and predicts a molecular mass of 51.8 kDa. As a result of glycosylation, the apparent molecular mass of rh IFNAR2/Fc monomer is approximately 65-75 kDa in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.