纯度

> 95 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

Immobilized Human IL-8/CXCL8 (His Tag)(Cat:10098-H01H1) at 2 μg/mL (100 μL/well) can bind Anti-IL-8/CXCL8 Antibody, Mouse Monoclonal(Cat:10098-MM18), the EC₅₀ is 16-90 ng/mL.

蛋白构建

A DNA sequence encoding the 77 amino acid endothelial-cell derived form ofthe mature human IL8 (NP_000575.1) (Ala 23-Ser 99) was fused with the Fc region of human IgG1 at the N-terminus.

NP No.

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Glu 20

分子量

The recombinant human Fc/IL8 (aa 1-77)chimera is a disulfide-linked homodimeric protein and the IL8 gene encodes the 1-77 amino acids. The reduced monomer consists of 314 amino acids and has a predicted molecular mass of 35.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh Fc/IL8 (aa 1-77) monomer is approximately 40 kDa due to glycosylation.

缓冲液

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.