纯度

> 90 % as determined by SDS-PAGE.

内毒素

< 1.0 EU per μg protein as determined by the LAL method.

生物活性

Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED₅₀ for this effect is 0.5-5ng/mL.

蛋白构建

A DNA sequence encoding the human IL23 complex composed of IL12B (NP_002178.2)(Met1-Ser328) and the mature form of human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was fused with a polyhistidine tag at the C-terminus.

NP No.

NP_002178.2 & NP_057668.1

表达宿主

HEK293 Cells

种属

Human

预测 N 端

Ile 23

分子量

The recombinant human IL23(IL23A&IL12B) consists of 502 amino acids and predicts a molecular mass of 55.8 kDa. The apparent molecular mass of human IL23 heterodimer is approximately 58.3 kDa respectively in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.