纯度

> 80 % as determined by SDS-PAGE

内毒素

< 1.0 EU per μg of the protein as determined by the LAL method

生物活性

1. Immobilized IL17RB Protein, Mouse, Recombinant (hFc Tag)(Cat:51109-M02H) at 2 μg/mL (100 μL/well) can bind IL25/IL17E Protein, Mouse, Recombinant (His Tag)(Cat:50138-M07H),the EC₅₀ is 4-20 ng/mL.
2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED₅₀ for this effect is 4-14ng/mL.

蛋白构建

A DNA sequence encoding the mature form of mouse IL25 (NP_542767.1) (Val 17-Ala 169) was expressed with a N-terminal polyhistidine tag.

NP No.

表达宿主

HEK293 Cells

种属

Mouse

预测 N 端

Gly

分子量

The secreted recombinant mouse IL25 consists of 175 amino acids and has a calculated molecular mass of 20 kDa. As a result of glycosylation, rmIL25 migrates with the MW of approximately 30-35 kDa in SDS-PAGE under reducing conditions.

缓冲液

Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

运输方式

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

稳定性 & 储存条件

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

复溶

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.