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产品编号: HYSSA008 产品名称:Anti-Human IgG-Fc (Biotin) 产品类型:/ 种属反应性:/ 应用:/

Overview
 

Description:

Goat polyclonal Secondary Antibody to Human IgG-Fc (Biotin).

Host

Goat

Target species:

Human IgG-Fc

Specificity:

By ELISA this antibody reacts specifically with Human IgG-Fc.
Has cross-reactivity in ELISA with Human IgG1, Human IgG2, Human IgG3, Human IgG4
May has cross-reactivity with the immunoglobulins of other species.

 

Has cross-reactivity with rat IgG (>10%), rabbit IgG (5.3%), horse IgG (4.1%)

Minimal cross-reactivity with mouse IgG, bovine IgG, guinea pig IgG

Applications:

Validated applications: ELISA
Expected applications: WB, IHC-P, ICC, IP
Please Note: Optimal concentrations/dilutions should be determined by the end user.

 
Properties

Form:

Liquid

Storage & Stability:

Store at +4℃ short term (1-2 weeks) (Preservative is recommended to avoid contamination). Upon delivery aliquot. Store at -20℃ to -80℃ for twelve months from date of receipt.Avoid repeated freeze-thaw cycles.

Constituents:

PBS

Purity:

Immunogen affinity purified

Purification notes:

Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

 
Applications

The application notes include recommended starting dilutions, optimal dilutions/concentrations should be determined by the end user.

 

IHC-P

0.2-5 μg/mL

ELISA

0.004-0.2 μg/mL

 WB

0.004-0.2 μg/mL

(Colormetric: 0.004-0.2 μg/mL; Chemiluminescent: 0.002-0.1 μg/mL)

IP

0.004-0.2 μg/mL

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